General Information

  • ID:  hor002303
  • Uniprot ID:  P56942
  • Protein name:  Melanin-concentrating hormone
  • Gene name:  Pmch
  • Organism:  Mus musculus (Mouse)
  • Family:  MCH family
  • Source:  Animal
  • Expression:  Expression is enhanced between postnatal days 10 and 15.|Predominantly expressed in hypothalamus. Also found in heart, intestine, spleen and testis (spermatogonia, early spermatocytes and Sertoli cells). In brain only mature MCH and NEI peptides are prese
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Mus (subgenus), Mus (genus), Murinae (subfamily), Muridae (family), Muroidea, Myomorpha (suborder), Rodentia (order), Glires, Euarchontoglires (superorder), Boreoeutheria, Eutheria, Theria, Mammalia (class), Amniota, Tetrapoda, Dipnotetrapodomorpha, Sarcopterygii (superclass), Euteleostomi, Teleostomi, Gnathostomata, Vertebrata, Craniata (subphylum), Chordata (phylum), Deuterostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0005102 signaling receptor binding; GO:0005179 hormone activity; GO:0030354 melanin-concentrating hormone activity; GO:0031777 type 1 melanin-concentrating hormone receptor binding
  • GO BP:  GO:0002027 regulation of heart rate; GO:0007204 positive regulation of cytosolic calcium ion concentration; GO:0007218 neuropeptide signaling pathway; GO:0007268 chemical synaptic transmission; GO:0007631 feeding behavior; GO:0032227 negative regulation of synaptic transmission, dopaminergic; GO:0042756 drinking behavior; GO:0045776 negative regulation of blood pressure; GO:0046005 positive regulation of circadian sleep/wake cycle, REM sleep; GO:0048168 regulation of neuronal synaptic plasticity
  • GO CC:  GO:0005576 extracellular region; GO:0005615 extracellular space; GO:0005634 nucleus; GO:0045202 synapse

Sequence Information

  • Sequence:  DFDMLRCMLGRVYRPCWQV
  • Length:  19(147-165)
  • Propeptide:  MAKMTLSSYMLMLAFSLFSQGILLSASKSIRNLEDDIVFNTFRMGKAFQKEDTAERSVVAPSLEQYKNDESGFMNDDDNKNSKNTGSKQNLVTHGLPLSLAVKPYLALKGSVAFPAENGVQNAESTQEKREIGDEENSAKFPIGRRDFDMLRCMLGRVYRPCWQV
  • Signal peptide:  MAKMTLSSYMLMLAFSLFSQG
  • Modification:  NA
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  MCH may act as a neurotransmitter or neuromodulator in a broad array of neuronal functions directed toward the regulation of goal-directed behavior, such as food intake, and general arousal.
  • Mechanism:  NA
  • Cross BBB:  YES
  • Target:  Mchr1
  • Target Unid:  Q8JZL2
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  45854
  • Structure ID:  AF-Q9VG55-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    AF-Q9VG55-F1.pdbhor002303_AF2.pdbhor002303_ESM.pdb

Physical Information

Mass: 271012 Formula: C105H162N30O26S4
Absent amino acids: AEHIKNST Common amino acids: R
pI: 8.22 Basic residues: 3
Polar residues: 4 Hydrophobic residues: 6
Hydrophobicity: -3.16 Boman Index: -3645
Half-Life: 1.1 hour Half-Life Yeast: 3 min
Half-Life E.Coli: >10 hour Aliphatic Index 71.58
Instability Index: 4108.95 Extinction Coefficient cystines: 7115
Absorbance 280nm: 395.28

Literature

  • PubMed ID:  8724342
  • Title:  Developmental and Stage-Dependent Expression of Melanin-Concentrating Hormone in Mammalian Germ Cells
  • PubMed ID:  8637571
  • Title:   A Role for Melanin-Concentrating Hormone in the Central Regulation of Feeding Behaviour